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Figure 2 | BMC Clinical Pathology

Figure 2

From: A simple and cost-effective method of DNA extraction from small formalin-fixed paraffin-embedded tissue for molecular oncologic testing

Figure 2

Primer extension assay for BRAF V600 mutation analysis. The primer extension assay was performed on a specimen that was shown to be positive for BRAF V600E mutation previously. A c.1799 T > A, p.V600E mutation was successfully identified using DNA extracted by the new method (Pinpoint solution was applied to H&E stained slides, followed by extraction using the Qiagen column, H-PQ). Red peak is the wild-type (nucleotide T) and green peak indicated by the arrow is the mutant peak (nucleotide A).

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