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Table 2 Synthetic spike-in levels in hemolysed samples

From: Pre-profiling factors influencing serum microRNA levels

Sample ID

cel-miR-39-3p raw Ct

cel-miR-39-3p raw Ct

unlysed sample

lysed sample

15

22.70

22.20

16

22.33

22.66

17

23.79

24.32

18

24.01

23.46

19

23.71

22.70

20

22.88

22.89

21

23.75

24.04

  1. The raw Ct values of a synthetic spiked-in RNA (cel-miR-39-3p) for all lysed and unlysed samples demonstrates that hemolysis did not have a significant impact on RNA isolation efficiency.