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Table 1 Primer and probe sequences used in the real-time quantitative polymerase chain reaction

From: Thymidine phosphorylase expression is associated with time to progression in patients with metastatic colorectal cancer

TP

Probe

5′-CAG CCA GAG ATG TGA CAG CCA CCG T-3′

 

forward primer

5′-CCT GCG GAC GGA ATC CTA TA-3′

 

reverse primer

5′-TGT GAT GAG TGG CAG GCT GT-3′

β-actin

probe

5′-CCT GAA CCC CAA GGC CAA CCG-3′

 

forward primer

5′-CGT GCT GCT GAC CGA GG-3′

 

reverse primer

5′-GAA GGT CTC AAA CAT GAT CTG GGT-3′

  1. Abbreviations: TP Thymidine Phosphorylase.