Figure 3

Grading of Ki-67 immunohistochemical staining on Spiral Array. Ki-67 positive ratio of tumor cells in a high power field (×400 magnification). Tumor cells with stained nucleus by the diaminobenzidine tetrahydrochloride, regardless their intensities, are count as positive. A) Scanning view of the Spiral Array slide stained with Ki-67. (bar = 5 mm). B-D) Examples of reels showing Ki-67 staining of Grade 3, > 30% (B); Grade 2, 11-30% (C); Grade 1, 1-10% (D). (bars in B, C, D = 100 μm).