| MDMX - | MDMX + | MDMX ++ | Total |
---|
 | P53 - | P53 + | P53 - | P53 + | P53 - | P53 + |
---|
MDM2 - | 9* | 2* | 0 | 0 | 0 | 0 | 11 |
MDM2 + | 3 | 1 | 4 | 0 | 0 | 2â€
|
10
|
MDM2 ++ | 4§
| 0 | 12§
| 1§
| 3â€
| 20â€
|
40
|
Total |
19
|
17
|
25
|
61
|
- Immunohistochemistry scores for MDM2 and MDMX were stratified as follows: (-) normal expression: when <11% of cells had positive nuclear staining; (+) moderate over-expression: when 11 – 40% of cells had positive nuclear staining; (++) strong over-expression: when >40% of cells had positive nuclear staining. Scores for P53 were: (-) negative expression: when ≤10% of cells had positive nuclear staining; (+) positive expression: when 11% or more of cells had positive nuclear staining.
- *MDM-P53 pathway is inactive as negative staining for MDM2 and MDMX; § collaborative MDM2 and MDMX pattern with higher MDM2 expression levels resulting in negative P53; †competitive MDM2 and MDMX pattern where comparable levels of MDM2 and MDMX expression lead to positive P53.