An observational study on the expression levels of MDM2 and MDMX proteins, and associated effects on P53 in a series of human liposarcomas

Table 2 P53 primers used in the study

Exon	Nucleotide sequence	Size	Tm°
P53 Exon 4	Forward: TCCCAAGCAATGGATGATTT	194 bp	63°C
P53 Exon 4	Reverse: TTCTGGGAAGGGACAGAAGA
P53 Exon 5٭	Forward: CTCTTCCTGCAGTACTCCCCTGC	211 bp	55°C
P53 Exon 5٭	Reverse: GCCCCAGCTGCTCACCATCGCTA
P53 Exon 6٭	Forward: GATTGCTCTTAGGTCTGGCCCCTC	182 bp	55°C
P53 Exon 6٭	Reverse: GGCCACTGACAACCACCCTTAACC
P53 Exon 7٭	Forward: GCTTGCCACAGGTCTCCCCAAG	192 bp	59°C
P53 Exon 7٭	Reverse: AGGCTGGCAAGTGGCTCCTGAC
P53 Exon 8٭	Forward: TGGTAATCTACTGGGACGGA	134 bp	50°C
P53 Exon 8٭	Reverse: GCTTAGTGCTCCCTGGGGGC
P53 Exon 9٭	Forward: GCCTCTTTCCTAGCACTGCCCAAC	102 bp	50°C
P53 Exon 9٭	Reverse: CCCAAGACTTAGTACCTGAAGGGTG

Tm°, actual annealing temperature used for each primer; ٭primers reproduced from reference [40].

ISSN: 1472-6890