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Figure 6 | BMC Clinical Pathology

Figure 6

From: A mill based instrument and software system for dissecting slide-mounted tissue that provides digital guidance and documentation

Figure 6

Applications. (A) qPCR analysis (using a mouse cyclin D1 gene amplicon) of cDNA prepared from RNA isolated from paraffinized FFPE mouse olfactory tissue. The same AOIs from serial tissue sections were either mesodissected using mineral oil, or manual (hand) dissected where the recovered tissue was deparaffinized in limonene and recovered by centrifugation (loss during the centrifugation steps probably account for the lower yield of the manual protocol). Two samples were isolated using each method and two qPCR reactions performed for each sample. Similar results were obtained using a mouse Bcl-2 gene amplicon (not shown). (B) FISH imaged fragments of tissue sections. FFPE tissue sections from human placenta (not shown: liver, bowel, and kidney performed similarly) were deparaffinized, mesodissected, and re-adhered to charged glass slides. These slides were processed using standard tissue FISH protocols and assayed with a chromosome 17 centromeric probe, then stained with DAPI. (C) Paraffinized tissue block milled with the mesodissection instrument.

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