Manual HER2 FISH (%) | Automated HER2 FISH (%) | Pearson chi2coefficient | P value | Concordance rate (%) | Cohen’s kappa | |
---|---|---|---|---|---|---|
HER2 genetic heterogeneity | ||||||
Present (5-50% carcinoma cells with HER2/CEP17 ratio >2.2) | 17 | 14 | 18.59 | < 0.001 | 85 | 0.428 |
Absent | 83 | 86 | ||||
HER2 cluster | ||||||
Present (HER2 cluster in >1% cells) | 41 | 39 | 76.71 | < 0.001 | 94 | 0.875 |
Absent | 59 | 61 | ||||
CEP17 polyploidy | ||||||
Present (Chr. 17 copy number >3.00) | 4 | 12 | 15.66 | < 0.001 | 90 | 0.335 |
Absent | 96 | 88 |