Figure 2

CTHRC1 detection by DAS-ELISA. A: Detection of the recombinant CTHRC1- and negative control protein (NFE2L3). B: Detection of the native CTHRC1 protein in colon cell lines HT29 and SW620, and E. coli protein extract used as negative control. The MAb CH21D7 was used as captured antibody and biotin-conjugated CH24G2 as detection antibody. The cellular extracts or control antigens were incubated in the wells during 1 h at 24°C or overnight at 4°C.