Figure 3

Immunohistochemical analysis of melanoma samples. Thirty-four melanoma tissues from a frozen TMA were stained for their expression of TNW and TNC. The area of the section stained for TNW or TNC was quantified and the samples were classified as in Figure 2. Representative pictures of each tumor type are grouped in different panels: A) primary tumors (PT), B) metastases (MET), C) lymph node metastases (LN-MET). From top to bottom, pictures in the left column of each panel represent increasing scores of TNW from absent, low, moderate to high (only in panel C) with the corresponding tumor stained for TNC in the right column of each panel. Panel E shows normal skin tissue stained for TNW (left) and TNC (right). Every picture is acquired at the same magnification (Scale bar = 200 μm). Table D summarizes the distribution of the scores for each tenascin in the different groups.