Effect of the filter number on macrophage capture by the LeukoCatch. (A) Human macrophage (MONO-MAC-6) cells were examined as a surrogate of leukocytes in the blood. Cells were subjected to serial passage (twice) of the same syringe (LeukoCatch system) that was equipped with 0, 1, 3, 5, 7 or 10 filters. NT signifies the non-treated blood sample. The "0" differs from NT because in "0", the blood sample was passed through the LeukoCatch system with a zero filer. The bar graphs represent the number of macrophage cells (flow-through) that were not captured with the filters. Cell counts were obtained by FACS (n = 4). (B) Western blot analysis by anti-GAPDH and anti-Erk1/2 antibodies using a 5-μL extract (10% of total) of cells escaped from or captured by the indicated numbers of filters in the LeukoCatch. (C) The bar graphs represent the relative intensity of the band signals measured by using the Image-J software, which were given as percentage of the starting material (NT: non-treated).