Table 2 Effect of frozen storage on the detection of bacterial OTUs by 16S rRNA-based T-RFLP
OTU (bp) | Log odds of detection | p value | 95% CI | Closest Bacterial Match (>97% Sequence Similarity) |
|---|---|---|---|---|
87 | 1.81 | 0.25 | -1.25, 4.86 | |
91 | -3.04 | 0.01 | -5.43, -0.65 | Firmicutes |
114 | -2.00 | 0.04 | -3.95, -0.05 | Moraxella sp. |
116 | 2.63* | 0.36 | -2.96, 8.22 | Marinomonas sp. |
133, 215 | 2.63* | 0.36 | -2.96, 8.22 | |
155 | 1.34 | 0.20 | -0.69, 3.38 | |
216 | -1.18 | 0.15 | -2.80, 0.43 | Corynebacterium propinquum, Rothia mucilaginosa |
273 | -2.44 | 0.26 | -6.66, 1.79 | Flavobacteriaceae |
294 | 0.94* | 0.52 | -1.89, 3.76 | |
309 | -2.77 | 0.03 | -5.34, -0.21 | Pseudomonas sp. |
392 | -2.21 | 0.06 | -4.52, 0.11 | |
492 | -0.67 | 0.57 | -2.99, 1.66 | Clostridiales Incertae Sedis XI |
502 | 2.63* | 0.36 | -2.96, 8.22 | |
504 | -2.39 | 0.25 | -6.49, 1.70 | Haemophilus influenzae |
518 | -2.59 | 0.01 | -4.58, -0.61 | Haemophilus influenzae, Burkholderia fungorum, Comamonadaceae |
520 | -0.36 | 0.68 | -2.02, 1.31 | Staphylococcus sp. |
521 | -1.70 | 0.11 | -3.80, 0.40 |