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Table 4 Comparison of Regular- and COLD-PCR in 61 FFPE Samples

From: COLD-PCR enhanced melting curve analysis improves diagnostic accuracy for KRAS mutations in colorectal carcinoma

   COLD-PCR  
   Pos Lo-Pos WT Total
Regular PCR Pos 19 0 0 19
  Lo-Pos 10 0 0 10
  WT 4 0 28 32
  Total: 33 0 28 61
  1. FFPE: Formalin-fixed paraffin-embedded Pos: Positive for KRAS mutation; Lo-Pos: Positive for KRAS mutation near the limit of detection; WT: Wild Type