Table 1 Primer and Probe Sequences (adapted from [11])

KRAS-F

5'-AAG GCC TGC TGA AAA TGA CTG-3'

Intron 1/Exon 2 boundary

25398312-25398332

KRAS-R

5'-GGT CCT GCA CCA GTA ATA TGC A-3'

Intron 2

25398168-25398189

Anchor Probe

5'-CGT CCA CAA AAT GAT TCT GAA TTA GCT GTA TCG TCA AGG CAC T-fluorescein-3'

Exon 2

25398228-25398270

Sensor Probe

5'-Cy5.5-TGC CTA CGC CAC CAG CTC CAA-phosphate-3'

Exon 2 spanning codons 12/13

25398273-25398293