TY - JOUR AU - Pritchard, Colin C. AU - Akagi, Laura AU - Reddy, Poluru L. AU - Joseph, Loren AU - Tait, Jonathan F. PY - 2010 DA - 2010/11/26 TI - COLD-PCR enhanced melting curve analysis improves diagnostic accuracy for KRAS mutations in colorectal carcinoma JO - BMC Clinical Pathology SP - 6 VL - 10 IS - 1 AB - KRAS mutational analysis is the standard of care prior to initiation of treatments targeting the epidermal growth factor receptor (EGFR) in patients with metastatic colorectal cancer. Sensitive methods are required to reliably detect KRAS mutations in tumor samples due to admixture with non-mutated cells. Many laboratories have implemented sensitive tests for KRAS mutations, but the methods often require expensive instrumentation and reagents, parallel reactions, multiple steps, or opening PCR tubes. SN - 1472-6890 UR - https://doi.org/10.1186/1472-6890-10-6 DO - 10.1186/1472-6890-10-6 ID - Pritchard2010 ER -