Lymph node infarction – a rare complication associated with disseminated intra vascular coagulation in a case of dengue fever

  • I Satish Rao1Email author,

    Affiliated with

    • Anand C Loya1,

      Affiliated with

      • KS Ratnakar1 and

        Affiliated with

        • VR Srinivasan2

          Affiliated with

          BMC Clinical Pathology20055:11

          DOI: 10.1186/1472-6890-5-11

          Received: 06 May 2005

          Accepted: 12 December 2005

          Published: 12 December 2005



          Lymph node infarction is known to occur in association with many non-neoplastic and neoplastic conditions however its occurrence in association with DIC is not reported hitherto in the literature.

          Case presentation

          We describe an unusual case of lymph node infarction in a twenty seven year old male following disseminated intravascular coagulation (DIC) in a case of dengue fever. Multiple sections of the infarcted and the surrounding non-infarcted lymph nodes failed to reveal any predisposing condition. How ever the parahilar vessels showed thrombotic occlusion, which must have been responsible for the infarction.


          Global infarction of the lymph node may mask the underlying pathology. Any malignancy especially lymphoma may coexist or follow lymph node infarction, therefore the patient needs constant surveillance.


          Lymph node infarction is a rare occurrence as it has abundant vascularity. Infarction can be due to various non-neoplastic and neoplastic conditions. It may frequently fore shadow neoplastic conditions especially lymphoma and a diligent search should be undertaken to identify the cause. We present a case of lymph node infarction detected in association with disseminated intravascular coagulation in a serologically proven case of Dengue fever.

          Case presentation

          This twenty-seven years old male presented with complaints of fever with rigor and chills and sore throat for ten days. It was associated with myalgia and recurrent vomiting followed by diffuse maculopapular rash all over the body with sparing of mucous membranes and periorbital tissues. On examination, he had conjunctival congestion with toxic facies. He had a past history of gout for which he received allolpurinol for twenty days and phenytoin for sudden loss of consciousness in another institution. There was no past history of tuberculosis, diabetes or hypertension.

          Bilateral hard indurated cervical lymphadenopathy and bilateral inguinal lymphadenopathy was also observed along with bilateral parotid and submandibular gland enlargement. Hepatosplenomegaly was detected on ultrasonography. Hemogram on several occasions from admission revealed normal hemoglobin and red blood cell morphology. There was transient neutrophilic leukocytosis, whereas platelet counts and erythrocyte sedimentation rate were normal throughout. During his treatment with ceftriaxone, he suddenly developed shock, cyanosis and acute renal failure for which he was given inotropic support, amoxicillin, ceftazidine and steroids. He gradually responded and recovered in due course of time. A clinical diagnosis of shock syndrome due to Dengue fever or Epstein Barr virus (EBV) or streptococcus or phenytoin induced hypersensitivity syndrome was made. The Dengue virus IgM antibody Elisa was positive and serum D-dimer levels were positive (>200 ng/ml). Anti EBV IgG antibody test was positive but EBV IgM antibody test was negative. The serology tests for anti- cytomegalovirus antibody, anti leptospira antibody, Widal test, brucella (abortus and melitensis), and HIV were negative. The blood, throat swab and urine cultures were sterile. Paul-Bunnel and Weil-Felix tests were negative. Cervical lymph node biopsy was undertaken for histopathology.

          Gross examination revealed four lymph nodes, the largest one measuring 1.5 cm and the rest below 1 cm. On microscopy, two of the lymph nodes (including the largest one) showed near total infarction of the parenchyma with preservation of cellular outlines of the lymphocytes (Fig 1). A thin rim of viable subcapsular normal mature lymphocytes along with neutrophils and plasma cells were identified. The medullary vessels showed vascular congestion however the blood vessels in the perinodal adipose tissue showed multiple fibrin thrombi with congestion in some of the vessels (Fig 2).

          Figure 1

          Lymph node infarction with viable rim of sub capsular lymphoid tissue. (40X, H & E) Inset: Note the cellular outlines of the individual lymphocytes, which have undergone coagulative necrosis. (400X, H&E).

          Figure 2

          Perinodal blood vessels show marked congestion (H&E-40X), and fibrin thrombi (Inset-H&E-100X).

          Multiple deeper sections revealed perinodal granulation tissue with viable subcapsular lymphoid tissue but no atypical cells, histiocytes or granulomas were seen. Stains for fungi and acid-fast bacilli were negative. The adjacent two uninvolved lymph nodes showed features of reactive change. Immunohistochemistry with B- cell (CD20) and T- cell (CD3) markers was performed. The viable lymphoid tissue showed an admixture of both B and T-cells. The infarcted tissue showed variable positivity and these markers highlighted few reactive follicles (Fig 3). The uninvolved lymph nodes also showed a normal positivity pattern.

          Figure 3

          CD20 immunohistochemistry highlights the viable subcapsular lymphoid tissue, where as the adjacent infarcted area also shows focal positivity (H&E-200X).

          The patient was asymptomatic thereafter and had complete regression of the lymph nodes. He was lost to follow up after two months of discharge from the hospital.

          Lymph node infarction is a rare phenomenon reported in literature in association with various neoplastic and non-neoplastic conditions. The non-neoplastic conditions associated with infarction are namely polyarteritis nodosa, viral infections (Parvovirus B19, Infectious mononucleosis), cholesterol atheromatous embolism, thrombosis, gold injections, intestinal volvulus, postmediastinoscopy, mononeuritis multiplex, fine needle aspiration cytology and finally may be idiopathic [111]. Hemorrhagic infarction of hilar lymph nodes has also been reported recently in association with heart lung transplantation. [1, 2]

          The neoplastic lesions most commonly associated with infarction are malignant lymphoma and metastatic melanoma [1, 1315]. The differential diagnosis pertaining to the present case would be the above mentioned two conditions along with necrotizing lymphadenitis (Kikuchi's lymphadenitis), mucocutaneous lymph node syndrome, and necrotizing granulomatous inflammation.

          Serial deeper sections failed to reveal any evidence of either lymphoma or melanoma, any granulomatous inflammation, and vasculitis or monocytoid histiocytes, thus ruling out the above mentioned conditions. Drug induced hypersensitivity reactions in a lymph node especially due to phenytoin are known in the form of inter follicular expansion by a mixed infiltrate of immunoblasts, plasma cells and eosinophils with varying degrees of vascular proliferation. Focal necrosis has also been observed. Other drugs have been reported to show reactive change [16]. These changes were not seen in the present case in the non-infarcted lymph nodes. In the present case it appears that infarction of the lymph node occurred before the DIC became clinically evident.

          A completely infarcted lymph node should alert the pathologist to the high possibility of malignant lymphoma, as it is the most common cause of spontaneous lymph node infarction [5]. Further investigation can be carried out by taking multiple deeper sections of the infarcted lymph node to visualize the viable subcapsular tissue for any clue of malignancy. Immunohistochemistry has been done on the infarcted lymph node sections to establish the underlying pathology. Although it is widely believed that necrotic tissue is not suitable for immunohistochemical study, this view may be inaccurate. [1720]

          The detection of spontaneous lymph node infarction should alert the pathologist and the clinician alike for an associated or underlying impending pathology. In a multicentre study of 51 cases of lymph-node infarction seen in the 30-year period, Maurer et al identified 14 cases of malignant lymphoma synchronously with the infarct. Of the remaining 37 patients, six showed manifestations of malignant lymphoma in the follow-up period within 2 years of the lymph-node infarction.

          On analyzing other cases in the literature these authors also confirmed that a minority (26 of 81) have developed malignant lymphoma, which appeared within 2 years of infarction. Therefore, a thorough examination of both the infarcted lymph nodes and others resected at the same time is mandatory in order to exclude concomitant or underlying malignant lymphoma. The risk of development of malignant lymphoma after infarction was found to be negligible after two years [15].


          We report a very unusual case of lymph node infarction associated with disseminated intra-vascular coagulation in dengue fever not yet reported in the literature. The simple reason may be that usually lymph nodes are not sampled in DIC. However, any infarcted lymph node should be eyed with suspicion especially when it is enlarged in size. Multiple deeper sections and immunohistochemistry may be very useful to unearth the underlying cause and finally when the cause is not found out, these patients should be followed up for a minimum of 2 years.

          List of abbreviations


          Disseminated intravascular coagulation


          Erythrocyte sedimentation rate


          Epstein Barr virus


          Immunoglobulin M


          Immunoglobulin G


          Human immunodeficiency virus


          Authors’ Affiliations

          Department of Pathology, Nizam's Institute of Medical Sciences
          Department of General Medicine, Nizam's Institute of Medical Sciences


          1. Rosai J: Lymph nodes, Chapter 21. Ackerman's Surgical Pathology 8 Edition (Edited by: Juan Rosai). Mosby – yearbook, Inc 1998, 2:1739–1741.
          2. Johnson LB, Pasumarthy A, Saravolatz LD: Parvovirus B19 infection presenting with necrotizing lymphadenitis. Am J Med 2003,114(4):340–341.View ArticlePubMed
          3. Kojima M, Nakamura S, Sugihara S, Sakata N, Masawa N: Lymph node infarction associated with infectious mononucleosis: report of a case resembling lymph node infarction associated with malignant lymphoma. Int J Surg Pathol 2002,10(3):223–226.View ArticlePubMed
          4. Shah KH, Kisilevsky R: Infarction of the lymph nodes: a cause of a palisading macrophage reaction mimicking necrotizing granulomas. Hum Pathol 1978,9(5):597–599.View ArticlePubMed
          5. Roberts C, Batstone PJ, Goodlad JR: Lymphadenopathy and lymph node infarction as a result of gold injections. J Clin Pathol 2001,54(7):562–564.View ArticlePubMed
          6. Mahy NJ, Davies JD: Ischaemic changes in human mesenteric lymph nodes. J Pathol 1984,144(4):257–267.View ArticlePubMed
          7. Miller RR, Nelems B: Mediastinal lymph node necrosis: a newly recognized complication of mediastinoscopy. Ann Thorac Surg 1989,48(2):247–250.View ArticlePubMed
          8. Tsuboi Y, Hayashi I, Hori T, Takahashi M, Yamada T: Subacute necrotizing lymphadenitis associated with mononeuritis multiplex- a case report. Rinsho Shinkeigaku 1999,39(4):465–467.PubMed
          9. Davies JD, Webb AJ: Segmental lymph-node infarction after fine-needle aspiration. J Clin Pathol 1982,35(8):855–857.View ArticlePubMed
          10. Tsang WY, Chan JK: Spectrum of morphologic changes in lymph nodes attributable to fine needle aspiration. Hum Pathol 1992,23(5):562–565.View ArticlePubMed
          11. Watts JC, Sebek BA, McHenry MC, Esselstyn CB Jr: Idiopathic infarction of intra-abdominal lymph nodes. A cause of fever of unknown origin. Am J Clin Pathol 1980,74(5):687–690.PubMed
          12. Ren H, Hruban RH, Baumgartner WA, Reitz BA, Baker RR, Hutchins GM: Hemorrhagic infarction of hilar lymph nodes associated with combined heart-lung transplantation. J Thorac Cardiovasc Surg 1990,99(5):861–867.PubMed
          13. Bhargava S, Tewari CP, Arun A: Lymph node infarction and lymphoma. Indian J Cancer 1989,26(4):233–239.PubMed
          14. Toriumi DM, Goldschmidt RA, Wolff AP: Lymph node infarction and malignant lymphoma: a case report. J Otolaryngol 1988,17(2):128–130.PubMed
          15. Maurer R, Schmid U, Davies JD, Mahy NJ, Stansfeld AG, Lukes RJ: Lymph-node infarction and malignant lymphoma: a multicentre survey of European, English and American cases. Histopathology 1986,10(6):571–588.View ArticlePubMed
          16. Cousar JB, Casey TT, Macon WR, McCurly TL, Swerdlow SH: Diagnostic surgical pathology 3 Edition (Edited by: Stephen S Sternberg). Philadelphia Lippincott Williams and Wilkins 1999, 2:727–728.
          17. Norton AJ, Ramsay AD, Isaacson PG: Antigen preservation in infarcted lymphoid tissue. A novel approach to the infarcted lymph node using monoclonal antibodies effective in routinely processed tissues. Am J Surg Pathol 1988,12(10):759–767.View ArticlePubMed
          18. Vega F, Lozano MD, Alcalde J, Pardo-Mindan FJ: Utility of immunophenotypic and immunogenotypic analysis in the study of necrotic lymph nodes. Virchows Arch 1999,434(3):245–248.View ArticlePubMed
          19. Nasuti JF, Gupta PK, Baloch ZW: Clinical implications and value of immunohistochemical staining in the evaluation of lymph node infarction after fine-needle aspiration. Diagn Cytopathol 2001,25(2):104–107.View ArticlePubMed
          20. Strauchen JA, Miller LK: Lymph node infarction. An immunohistochemical study of 11 cases. Arch Pathol Lab Med 2003,127(1):60–63.PubMed
          21. Pre-publication history

            1. The pre-publication history for this paper can be accessed here:http://​www.​biomedcentral.​com/​1472-6890/​5/​11/​prepub


          © Rao et al. 2005

          This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://​creativecommons.​org/​licenses/​by/​2.​0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.