Clinical chemistry profiles in injection heroin users from Coastal Region, Kenya
© Were et al.; licensee BioMed Central Ltd. 2014
Received: 5 March 2014
Accepted: 26 June 2014
Published: 9 July 2014
Although the co-burden of injection drug use and HIV is increasing in Africa, little is known about the laboratory markers of injection drug use and anti-retroviral treatment (ART) in Kenyan injection drug users. This study, therefore, aimed at determining the clinical chemistry profiles and identifying the key laboratory markers of HIV infection during ART in injection heroin users (IHUs).
Clinical chemistry measurements were performed on serum samples collected from HIV-1 infected ART-experienced (n = 22), naive (n = 16) and HIV-1 negative (n = 23) IHUs, and healthy controls (n = 15) from Mombasa, coastal Kenya.
HIV uninfected IHUs had lower alanine aminotransferase (ALT) levels (P = 0.023) as ART-exposed IHUs exhibited lower albumin (P = 0.014) and higher AST to platelet index (APRI) (P < 0.0001). All IHUs presented with lower aspartate aminotransferase to ALT values (P = 0.001) and higher C-reactive protein (CRP) levels (P = 0.002). ART-naive IHUs had higher globulin levels (P = 0.013) while ART-experienced and naive IHUs had higher albumin to total protein (P < 0.0001) and albumin to globulin (P < 0.0001) values. In addition, CD4+ T cells correlated with ALT (ρ = −0.522, P = 0.011) and CRP (rho, ρ = 0.529, P = 0.011) in HIV negative and ART-experienced IHUs, respectively. HIV-1 viral load correlated with albumin to globulin index in ART-experienced (ρ = −0.468, P = 0.037) and naive (ρ = −0.554, P = 0.040) IHUs; and with albumin to total protein index (ρ = −0.554, P = 0.040) and globulin (ρ = 0.570, P = 0.033) in ART-naive IHUs.
Absolute ALT, albumin, globulin, and CRP measurements in combination with APRI, AST to ALT, albumin to total protein and albumin to globulin indices may be useful laboratory markers for screening IHUs for initiating and monitoring treatment.
KeywordsInjection heroin user Clinical chemistry markers HIV-1 infection Anti-retroviral treatment
Of the 14 million people who inject drugs worldwide, 1.64 million are HIV infected . In addition, more than 40% of new HIV infections in the world are attributed to injection drug use . HIV sero-prevalence rates among injection drug users (IDUs) in Kenya are exceedingly high at 18%, and at least, 17% of new HIV infections in the country are linked to injection drug use . Moreover, the rates of HIV transmission in injection drug users in the country are higher relative to the spread in the general population , suggesting that injection drugs and substance use are important in promoting the spread of HIV in Kenya.
Although CD4+ T cell measurements are commonly used in initiating and monitoring disease progression and treatment in HIV-1 infected individuals , other biomarkers may not be specific to HIV infection due to concomitant illicit drug and poly-substance use among injection drug users. Since, addictive drugs such as opioids largely cause persistent immune stimulation inducing chronic inflammation , while anti-retroviral therapy cumulatively cause hepatotoxic injury , it is likely that HIV-1 infected injection drug users progressively develop intense inflammation and hepatotoxicity that can be indirectly assessed through determining hepatic functionality.
Injection drugs usually cause chronic liver degeneration and marked derangements in hepatic synthetic functions [6, 8, 9]. Elevated alanine aminotransferase (ALT), aspartate aminotransferase (AST), globulin, aspartate aminotransferase to platelet index (APRI), and C-reactive protein (CRP) levels are linked to injection drug use in both HIV infected and uninfected individuals [10–14]. In contrast, addictive heroin doses promote reductions in hepatic albumin synthesis . Taken together, these studies suggest increased inflammatory-mediated hepatic derangements in both HIV infected and uninfected injection drug users.
However, no studies to date have investigated clinical chemistry profiles in injection heroin users, resident at coastal Mombasa, Kenya. The identification of clinical chemistry laboratory markers in this sub-population is of great importance, since it can assist in the clinical management of HIV in injection drug users. The current study, therefore, examined clinical chemistry laboratory markers in HIV-1 infected ART-experienced, naïve and HIV-1 non-infected injection heroin users, and healthy controls.
Study site and population
This cross-sectional clinical laboratory study was conducted as part of a larger study investigating the microbiological and immunological determinants of HIV infection among adult injection drug users. The study was undertaken at Bomu Hospital, a social enterprise facility in Mombasa, a coastal region of Kenya. Mombasa has a large injection drug using population of about 26,000, with heroin being the predominant injection drug . Injection drug users were recruited via respondent driven sampling, snowball and makeshift outreach methods. Only individuals (age ≥18 yrs.) exhibiting needle scars, reporting injection heroin use at least once in the previous month and providing written informed consent were recruited into the study. Healthy controls were recruited from among HIV negative individuals presenting no evidence of illness and history of drug use.
From each injection drug user and healthy control, 10 ml of venous blood was collected. Of this, 5 ml was placed into both the EDTA and plain BD vacutainer® tubes (BD, Franklin Lakes, USA). The EDTA blood samples were used immediately after collection for hematological measurements and the enumeration of CD4+ T cells. Serum was obtained from clotted blood after clot retraction and centrifugation at 15,000 r.p.m for 10 minutes. The serum samples were used for the determinations of clinical chemistry analytes, HIV and hepatitis virus sero-testing, and viral loads.
HIV-1 testing was performed using rapid immunochromatographic tests, Determine™ (Abbott Laboratories, Tokyo, Japan) and Unigold™ (Trinity Biotech Plc, Bray, Ireland). Study participants with positive results for both Determine and Unigold were considered HIV infected based on the Kenyan national HIV testing algorithm .
HBV and HCV testing
Sero-diagnosis for the presence of hepatitis B virus (HBV) and hepatitis C virus (HCV) infections was performed using the one-step HBV-5 panel, and anti-HCV rapid diagnostic tests, respectively (Healthaw Medical limited, Hangzhou, China). Serum samples were tested for sero-reactivity against the HBV panel markers and anti-HCV. HBsAg and anti-HCV reactive individuals were considered sero-positive for HBV and/or HCV infection.
Clinical chemistry measurements
Direct clinical chemistry determinations were performed for ALT, AST, gamma-glutamyl transpeptidase (GGT), total protein, albumin, creatinine, urea, total cholesterol, high density lipoprotein (HDL), CRP, phosphocreatine kinase isoenzymes (CK-MB), and vitamin D using the automated clinical chemistry analyzer (Roche COBAS® 6000, Lausanne, Switzerland). Globulin was calculated by subtracting albumin concentrations from the total protein levels and the albumin to globulin ratio >1 was considered normal . The reference values were based on our laboratory established reference ranges. APRI was determined as previously described , with AST upper limit values from laboratory established reference values (<37 U/L).
CD4+ T cell enumerations
Baseline CD4+ T cell counts were determined in an automated fashion using the BD FACSCalibur flow cytometer (Becton-Dickinson™, Franklin Lakes, USA). Briefly, 5.0 μl of EDTA blood samples were placed in a tube and RBC lysis buffer added. After 5 minute incubation, the cells were washed and fluorescent-tagged antibodies (anti-CD3, anti-CD4, and anti-CD45) were added. The cells were incubated for 30 minutes after which the samples were washed and the CD4+ T cells enumerated on the flow cytometer.
HIV-1 viral load
HIV-1 viral loads were determined using the automated Abbott m2000 System according to the manufacturer’s instructions (Abbott Molecular Inc., Illinois, USA). Briefly, RNA was extracted from 0.2 ml serum samples and reverse-transcribed into cDNA. The cDNA was amplified using HIV-1-specific and internal control primers. Fluorescence intensity of the HIV-1 probe was converted into viral loads by the analyzer.
Ethical approval for the study was obtained from Kenyatta University Ethical Review Committee. Each participant gave written informed consent prior to enrolment into the study. Confidentiality of the study participant’s information was ensured throughout the study by coding and limiting accessibility of the study information. The study participants benefited from free health education on HIV, tuberculosis (TB), hepatitis B and C, sexually transmitted infections, hygiene and nutrition. HIV-positive, TB-positive and HIV-TB co-infected individuals were referred to the comprehensive care centers at Bomu Hospital or the Coast General Provincial Hospital for treatment, support and care.
Statistical analysis was conducted using IBM® SPSS Statistics 19.0 (SPSS Inc. Chicago, USA). Continuous data (age, and laboratory measures) summarized as medians (IQR) and categorical data (gender, duration of injection, and non-injection drugs) presented as proportions were tabulated. Differences in the proportions were determined using the chi-square tests. Statistical comparisons of the continuous data across the study groups were performed using non-parametric ANOVA (Kruskal Wallis) tests followed by Bonferroni post-hoc corrections for multiple comparisons. Spearman’s rank correlation tests were used to determine the associations of clinical chemistry measures with CD4+ T cells and viral loads within the study groups. A two-sided probability value <0.05 was considered statistically significant. Bonferroni correction for multiple comparisons were determined by dividing the criterion P < 0.05 by the total number of the study groups (P < 0.05/4 = P < 0.0125). The P < 0.0125 was then used for statistical inferences following between-group Mann Whitney U comparisons.
Demographic and laboratory characteristics of the study participants
Demographic, clinical and laboratory characteristics of the study participants
HC, n = 15
IHUs n = 23
ART-naive IHUs, n = 16
ART-exposed IHUs, n = 22
Injection drugs, n (%)
Duration of injection, n (%)
Non-injection drugs, n (%)
CD4+ T cell count/μl
Viral loads, copies/μl
Clinical chemistry measurements
While across group analysis did not show significant differences in the levels of phosphocreatine kinase isoenzymes (CK-MB) (P = 0.964), the levels of CRP differed significantly across the study groups (P = 0.002). Subsequent post-hoc corrections revealed significantly higher levels of CRP in anti-retroviral treatment-exposed (median, 4.0 mg/L; IQR, 7.8; P = 0.001), anti-retroviral treatment-naive (median, 3.8 mg/L; IQR, 14.2; P < 0.0001) and in HIV-1 uninfected injection heroin users (median, 6.1 mg/L; IQR, 8.4; P = 0.001) in comparison to healthy controls (median, 0.7 mg/L; IQR, 0.9) signifying the importance of CRP as an inflammation marker of injection heroin use.
Clinical chemistry measurements of the study participants
HC, n = 15
IHUs, n = 23
ART-naive IHUs, n = 16
ART-exposed IHUs, n = 22
CRP > 5.0 mg/L, n (%)
M > 40; F > 31, n (%)
AST/ALT ≥2.0, n (%)
APRI ≥0.5, n (%)
ALB <32.0 g/L, n (%)
Total PROT, g/L
ALB/GLB ≤1, n (%)
Vitamin D, ng/ml
AST, ALT, albumin, globulin and platelet ratios
Values for the AST to ALT ratio varied across the groups (P = 0.001) and were higher in the anti-retroviral treatment-exposed injection heroin users (median, 1.6; IQR, 0.8; P < 0.01), anti-retroviral treatment-naive injection heroin users (median, 2.1; IQR, 0.9; P < 0.01) and HIV negative injection heroin users (median, 1.8; IQR, 1.0; P < 0.01) relative to healthy controls (median, 1.1; IQR, 0.7). In addition, at least a quarter of the anti-retroviral treatment-exposed (27.3%), treatment-naive (62.5%) and HIV negative injection heroin users (43.5%) had AST to ALT ratio ≥2.0, indicating increased liver damage in both HIV infected and uninfected injection heroin users.
Albumin to total protein ratio differed in the groups (P < 0.0001) and were significantly lower in the anti-retroviral treatment-exposed (median, 0.5; IQR, 0.1) and treatment-naive (median, 0.4; IQR, 0.2) groups compared to HIV negative injection heroin users (median, 0.6; IQR, 0.1) and healthy controls (median, 0.6; IQR, 0.1; P < 0.01 and P < 0.0001, respectively). Likewise, albumin to globulin ratio varied in the study groups (P < 0.0001) such that the anti-retroviral treatment-experienced (median, 1.0; IQR, 0.5) and naive (median, 0.8; IQR, 0.6) individuals had lower values in comparison to HIV negative group (median, 1.5; IQR, 0.4; P = 0.002) and healthy controls (median, 1.4; IQR, 0.3; P < 0.0001).
APRI differed significantly across the study groups (P = 0.005) with the anti-retroviral treatment-exposed group (median, 0.25; IQR, 0.20) having higher values compared to the healthy controls (median, 0.14; IQR, 0.10; P = 0.001). Moreover, the frequency of moderate-to-severe forms (i.e., APRI ≥ 0.5) of liver damage was only noted in anti-retroviral treatment-exposed (18.2%) and -naive (6.3%) individuals (Table 2), suggesting increased liver derangements in HIV infected injection heroin users.
Correlation of the clinical chemistry markers with CD4+ T cells and HIV viral loads
Clinical chemistry laboratory analysis constitutes a key step in patient assessment for initiating and monitoring response to anti-retroviral treatment . The reliability and accuracy of clinical chemistry analytes, however, is confounded by complex interaction between injection drug use and anti-retroviral drugs. Therefore, this cross-sectional clinical laboratory study determined the clinical chemistry markers in HIV infected and uninfected injection heroin users from coastal Kenya.
Absolute albumin levels and albumin to total protein ratio reductions observed in the HIV-1 infected injection drug users are indications of derangements in protein metabolism. The decreases in the albumin levels can be attributed to low dietary intake of proteins that is linked to low synthesis of albumin , a feature of malnutrition that is frequently observed among injection drug users presenting with and without HIV infection . It is also possible that suppression of albumin results from inhibition of hepatic synthesis at abusive doses of heroin . In addition, opioid-induced hepatotoxicities and inflammatory-mediated hepatic damage cause reduced hepatic synthetic functions [15, 21]. Thus, findings presented here suggest that injection heroin use among HIV-infected individuals promote increased reductions in the albumin levels and marked alterations in the albumin to total protein ratio.
The inverse associations of HIV viral load and albumin to total protein and albumin to globulin indices in the anti-retroviral-naive injection heroin users signify disease progression but during treatment the albumin to globulin ratio is inverted indicating reductions in the viral loads and immune reconstitution. This premise is, in part, consistent with clinical studies in HIV infected patients commencing highly active anti-retroviral therapy showing that hypoalbuminaemia is associated with morbidity and mortality [22, 23]. Therefore, reduced albumin and low albumin to total protein ratio are important laboratory measures in injection heroin users that can be utilized as important surrogates for screening and for initiating and monitoring of anti-retroviral treatment in HIV infected injection heroin users.
In contrast to previous studies, a higher globulin level was found in the HIV infected injection heroin users naive for anti-retroviral treatment . This finding suggests that hyperglobulinaemia is an important laboratory marker of HIV infected patients injecting illicit drugs. Hyperglobulinaemia characterizes B cell dysfunction during primary HIV infections and persisting into chronic infections . The lower median albumin to globulin values in the presence of higher proportions of ratios ≤1.0, and the correlations of HIV viral loads with globulin in the anti-retroviral treatment-naive individuals, further corroborate increased derangements in B cell functions in both HIV infected and uninfected injection heroin users. The findings of the current study also parallel studies showing elevated globulin and the albumin to globulin ratio in Australian drug addicts . In addition, elevations in total IgM and IgG, specific anti-morphine IgM, and cross-reactive IgM auto-antibodies were recorded in HIV infected and uninfected opioid users [25–27]. Hence, it appears heroin-induced antibodies promote inflammation leading to liver damage in injection users. Elevated globulin with concomitant lower albumin to globulin ratio may thus be a useful biomarker for clinical laboratory assessment of HIV infected and uninfected injection heroin users.
Reduced absolute ALT levels found in the HIV sero-negative injection heroin users, indicate reduced hepatic functionality. However, the higher AST to ALT ratio and proportions of AST to ALT ratio ≥2.0 in the HIV sero-negative and infected injection heroin users naive or on anti-retroviral treatment, suggest synergistic heroin and anti-retroviral drug-induced hepatic inflammation. These results are similar to observations showing that the AST to ALT ratio is a better measure of liver enzyme activity . In addition, the results are, in part, supported by the elevated AST, AST to ALT ratios, and higher proportions of ALT observed in patients on anti-retroviral treatment [10–12]. Since a large number of the injection heroin users in the present study were concomitantly consuming alcohol and khat, it is possible that these non-injection substances synergistically promote the alterations in the ALT levels [10, 29]. Importantly, the inverse correlations of absolute ALT levels and the CD4+ T cell counts in the HIV sero-negative injection heroin users, suggests that the CD4+ T cell count can also be utilized as a surrogate marker of liver function in the management of HIV negative heroin users.
The greater magnitude APRI median values and proportions ≥0.5 in the HIV infected injection heroin users on anti-retroviral treatment, suggests, that APRI is a better screening indicator of heroin and anti-retroviral treatment in this population. This observation is, in part, parallel to the time-dependent increases and prognostic value of APRI among HIV and hepatitis C co-infected patients undergoing anti-retroviral treatment , and studies in injection drug users showing the utility of APRI in predicting hepatic fibrosis . It is, therefore, likely that increased hepatotoxicity in HIV patients on anti-retroviral treatment results from the synergistic effect of anti-retroviral drugs, heroin and poly-substance consumption. Hence, APRI and proportions of APRI ≥0.5 are important measures for evaluating the degree of hepatoxicity in HIV infected injection heroin users undergoing anti-retroviral treatment.
Elevations of the C-reactive protein in all the study groups of injection heroin users is similar to previous studies showing elevated C-reactive protein in Australian drug addicts , and higher C-reactive protein levels in buprenorphine injection users in Singapore . Higher levels and proportions of individuals of this acute phase protein in both HIV infected and uninfected injection heroin users in the present study are indicative of increased liver synthesis following consumption of the illicit drugs, infection and cytokine release . In addition, the increases indicate heightened liver damage through illicit drug- and anti-retroviral treatment-mediated necrosis, apoptosis, and immune mechanisms. Of significance are analyses showing positive correlations between the C-reactive protein levels and the CD4+ T cell count in injection heroin users on anti-retroviral treatment. This result may reflect development of immune reconstitution inflammatory syndrome (IRIS) in the injection heroin users on anti-retroviral treatment resulting from poor adherence and illicit drug use. Consistent with this hypothesis, previous studies illustrated a link between IRIS and low adherence to anti-retroviral therapy, alcohol use, and low suppression of viral load among South African adults initiating anti-retroviral therapy . Implications of this observation include utilization of the C-reactive protein in assessing immune reconstitution following initiation of anti-retroviral treatment in injection heroin users.
Our results clearly show high rates of poly-drug use among injection heroin users in Mombasa. It is highly likely that the complex interactions of opioids and/or active compounds in bhang (Δ9-tetrahydrocannabinol), cigarettes (nicotine), rohypnol (benzodiazepines), alcohol, khat (cathinone), and anti-retroviral drugs promote the occurrence of drug dependence and adverse events. With regards to clinical chemistry profiles, such complex interactions induce hepatic metabolic derangements leading to toxicity and altered profiles of clinical chemistry markers. Consistent with these propositions, previous studies among drug users showed that interactions between opioids and benzodiazepines or alcohol increase occurrence of adverse events, overdose and death , as nicotine increase rates of opioid consumption . In addition, drugs of abuse (alcohol, opioids, benzodiazepines, marijuana, and nicotine) reduce the efficacy of anti-retroviral drugs leading to toxicity, treatment failure and high viral loads . Taken together, poly-drug use appears to promote toxicity leading to altered clinical chemistry profiles in both HIV-1 infected and uninfected injection drug users.
While a prospective design would have been important in examining the utility of clinical chemistry markers, in response to drug rehabilitation and HIV treatment, this cross-sectional study provides the first baseline information for screening of injection drug users for initiating and monitoring anti-retroviral treatments in Kenya. Although recruitment into this study was based on self-reported injection heroin use, it is possible that the study participants were also using other opioids, hence the need to carry out toxicological analyses to provide additional insights into the complex interactions between injection drugs and anti-retroviral treatment. These collectively can be linked to clinical laboratory markers and patient prognosis in a prospective approach.
This study provides further evidence that chronic inflammation in HIV infected and uninfected injection heroin users is characterized by derangements in hepatic synthetic functions. Our data suggest that injection heroin, HIV infection and anti-retroviral treatment differentially alter ALT; albumin; APRI and AST to ALT, albumin to total protein and albumin to globulin indices; C-reactive protein; and globulin in injection drug users.
We thank the study participants for making this study possible. We are grateful to the management and staff of the Bomu Hospital for their support during the study. This study was supported, in part, by the Kenya National Commission for Science, Technology and Innovation [NCST/5/003/065], and Partnership for Innovative Medical Education in Kenya (NIH 1R24TW008889) grants to TW and VB.
- UNODC: World Drug Report 2013. 2013, New York: The United Nations Office on Drugs and CrimeView ArticleGoogle Scholar
- UNAIDS: Global Report: UNAIDS Report on the Global AIDS Epidemic 2013. 2013, New York: Joint United Nations Programme on HIV/AIDSGoogle Scholar
- NASCOP: Rapid Situational Assessment of HIV Prevalence and Related Risky Behaviours Among Injecting Drug Users in Nairobi and Coast Provinces of Kenya. 2012, Kenya: National AIDS and STI Control Programme, Ministry of HealthGoogle Scholar
- NASCOP: Kenya AIDS Indicator Survey 2012 Report. 2013, Kenya: National AIDS and STI Control Programme, Ministry of HealthGoogle Scholar
- NASCOP: Guidelines for Antiretroviral Therapy in Kenya 2011. 2012, Kenya: National AIDS and STI Control Programme, Ministry of HealthGoogle Scholar
- Reece AS: Chronic immune stimulation as a contributing cause of chronic disease in opiate addiction including multi-system ageing. Med Hypotheses. 2010, 75 (6): 613-619.View ArticlePubMedGoogle Scholar
- Moodie EE, Pant Pai N, Klein MB: Is antiretroviral therapy causing long-term liver damage? A comparative analysis of HIV-mono-infected and HIV/hepatitis C co-infected cohorts. PLoS One. 2009, 4 (2): e4517-View ArticlePubMedPubMed CentralGoogle Scholar
- Chen CK, Su LW, Lin SK: Characteristics of hospitalized heroin smokers and heroin injectors in Taiwan. Changgeng Yi Xue Za Zhi. 1999, 22 (2): 197-203.PubMedGoogle Scholar
- Weller IV, Cohn D, Sierralta A, Mitcheson M, Ross MG, Montano L, Scheuer P, Thomas HC: Clinical, biochemical, serological, histological and ultrastructural features of liver disease in drug abusers. Gut. 1984, 25 (4): 417-423.View ArticlePubMedPubMed CentralGoogle Scholar
- Drumright LN, Hagan H, Thomas DL, Latka MH, Golub ET, Garfein RS, Clapp JD, Campbell JV, Bonner S, Kapadia F, Thiel TK, Strathdee SA: Predictors and effects of alcohol use on liver function among young HCV-infected injection drug users in a behavioral intervention. J Hepatol. 2011, 55 (1): 45-52.View ArticlePubMedGoogle Scholar
- Langohr K, Sanvisens A, Fuster D, Tor J, Serra I, Rey-Joly C, Rivas I, Muga R: Liver enzyme alterations in HCV-monoinfected and HCV/HIV-coinfected patients. Open AIDS J. 2008, 2: 82-88.View ArticlePubMedPubMed CentralGoogle Scholar
- Reece AS: Evidence of accelerated ageing in clinical drug addiction from immune, hepatic and metabolic biomarkers. Immun Ageing. 2007, 4: 6-View ArticlePubMedPubMed CentralGoogle Scholar
- Mehta SH, Vogt SL, Srikrishnan AK, Vasudevan CK, Murugavel KG, Saravanan S, Anand S, Kumar MS, Ray SC, Celentano DD, Solomon S, Solomon SS: Epidemiology of hepatitis C virus infection & liver disease among injection drug users (IDUs) in Chennai, India. Indian J Med Res. 2011, 132: 706-714.Google Scholar
- Samikkannu T, Rao KV, Arias AY, Kalaichezian A, Sagar V, Yoo C, Nair MP: HIV infection and drugs of abuse: role of acute phase proteins. J Neuroinflammation. 2013, 10: 113-View ArticlePubMedPubMed CentralGoogle Scholar
- Gomez-Lechon MJ, Ponsoda X, Jover R, Fabra R, Trullenque R, Castell JV: Hepatotoxicity of the opioids morphine, heroin, meperidine, and methadone to cultured human hepatocytes. Mol Toxicol. 1987, 1 (4): 453-463.PubMedGoogle Scholar
- NASCOP: Guidelines for HIV Testing and Counselling and Kenya. 2010, Kenya: National AIDS and STI Control Programme, Ministry of HealthGoogle Scholar
- Serpa J, Haque D, Valayam J, Breaux K, Rodriguez-Barradas MC: Effect of combination antiretroviral treatment on total protein and calculated globulin levels among HIV-infected patients. Int J Infect Dis. 2010, 14 (Suppl 3): e41-e44.View ArticlePubMedGoogle Scholar
- Wai CT, Greenson JK, Fontana RJ, Kalbfleisch JD, Marrero JA, Conjeevaram HS, Lok AS: A simple noninvasive index can predict both significant fibrosis and cirrhosis in patients with chronic hepatitis C. Hepatology. 2003, 38 (2): 518-526.View ArticlePubMedGoogle Scholar
- Kaysen GA, Yeun J, Depner T: Albumin synthesis, catabolism and distribution in dialysis patients. Miner Electrolyte Metab. 1997, 23 (3–6): 218-224.PubMedGoogle Scholar
- Forrester JE: Nutritional alterations in drug abusers with and without HIV. Am J Infect Dis. 2006, 2 (3): 173-179.View ArticlePubMedPubMed CentralGoogle Scholar
- Valente MJ, Carvalho F, Bastos M, de Pinho PG, Carvalho M: Contribution of oxidative metabolism to cocaine-induced liver and kidney damage. Curr Med Chem. 2012, 19 (33): 5601-5606.View ArticlePubMedGoogle Scholar
- Dao CN, Peters PJ, Kiarie JN, Zulu I, Muiruri P, Ong’ech J, Mutsotso W, Potter D, Njobvu L, Stringer JS, Borkowf CB, Bolu O, Weidle PJ: Hyponatremia, hypochloremia, and hypoalbuminemia predict an increased risk of mortality during the first year of antiretroviral therapy among HIV-infected Zambian and Kenyan women. AIDS Res Hum Retroviruses. 2011, 27 (11): 1149-1155.View ArticlePubMedGoogle Scholar
- Sudfeld CR, Isanaka S, Aboud S, Mugusi FM, Wang M, Chalamilla GE, Fawzi WW: Association of serum albumin concentration with mortality, morbidity, CD4 T-cell reconstitution among Tanzanians initiating antiretroviral therapy. J Infect Dis. 2013, 207 (9): 1370-1378.View ArticlePubMedPubMed CentralGoogle Scholar
- Titanji K, Chiodi F, Bellocco R, Schepis D, Osorio L, Tassandin C, Tambussi G, Grutzmeier S, Lopalco L, De Milito A: Primary HIV-1 infection sets the stage for important B lymphocyte dysfunctions. AIDS. 2005, 19 (17): 1947-1955.View ArticlePubMedGoogle Scholar
- Blanck RR, Ream N, Deegan MJ: Immunoglobulins in heroin users. Am J Epidemiol. 1980, 111 (1): 81-86.PubMedGoogle Scholar
- Gamaleya N, Tagliaro F, Parshin A, Vrublevskii A, Bugari G, Dorizzi R, Ghielmi S, Marigo M: Immune response to opiates: new findings in heroin addicts investigated by means of an original enzyme immunoassay and morphine determination in hair. Life Sci. 1993, 53 (2): 99-105.View ArticlePubMedGoogle Scholar
- McGowan JP, Shah SS, Small CB, Klein RS, Schnipper SM, Chang CJ, Rosenstreich DL: Relationship of serum immunoglobulin and IgG subclass levels to race, ethnicity and behavioral characteristics in HIV infection. Med Sci Monit. 2006, 12 (1): CR11-CR16.PubMedGoogle Scholar
- Sorbi D, Boynton J, Lindor KD: The ratio of aspartate aminotransferase to alanine aminotransferase: potential value in differentiating nonalcoholic steatohepatitis from alcoholic liver disease. Am J Gastroenterol. 1999, 94 (4): 1018-1022.View ArticlePubMedGoogle Scholar
- Roelandt P, George C, D’Heygere F, Aerts R, Monbaliu D, Laleman W, Cassiman D, Verslype C, Van Steenbergen W, Pirenne J, Wilmer A, Nevens F: Acute liver failure secondary to khat (Catha edulis)-induced necrotic hepatitis requiring liver transplantation: case report. Transplant Proc. 2011, 43 (9): 3493-3495.View ArticlePubMedGoogle Scholar
- Wilson LE, Torbenson M, Astemborski J, Faruki H, Spoler C, Rai R, Mehta S, Kirk GD, Nelson K, Afdhal N, Thomas DL: Progression of liver fibrosis among injection drug users with chronic hepatitis C. Hepatology. 2006, 43 (4): 788-795.View ArticlePubMedGoogle Scholar
- Ho RC, Ho EC, Mak A: Cutaneous complications among i.v. buprenorphine users. J Dermatol. 2009, 36 (1): 22-29.View ArticlePubMedGoogle Scholar
- Lobo SM: Sequential C-reactive protein measurements in patients with serious infections: does it help?. Crit Care. 2012, 16 (3): 130-View ArticlePubMedPubMed CentralGoogle Scholar
- Nachega JB, Morroni C, Chaisson RE, Goliath R, Efron A, Ram M, Maartens G: Impact of immune reconstitution inflammatory syndrome on antiretroviral therapy adherence. Patient Prefer Adherence. 2012, 6: 887-891.View ArticlePubMedPubMed CentralGoogle Scholar
- Gudin JA, Mogali S, Jones JD, Comer SD: Risks, management, and monitoring of combination opioid, benzodiazepines, and/or alcohol use. Postgrad Med. 2013, 125 (4): 115-130.View ArticlePubMedPubMed CentralGoogle Scholar
- Spiga R, Schmitz J, Day J: Effects of nicotine on methadone self-administration in humans. Drug Alcohol Dep. 1998, 50 (2): 157-165.View ArticleGoogle Scholar
- Pal D, Kwatra D, Minocha M, Paturi DK, Budda B, Mitra AK: Efflux transporters- and cytochrome P-450-mediated interactions between drugs of abuse and antiretrovirals. Life Sci. 2011, 88 (21–22): 959-971.View ArticlePubMedGoogle Scholar
- The pre-publication history for this paper can be accessed here:http://www.biomedcentral.com/1472-6890/14/32/prepub
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